Journal
ADVANCES IN NATURAL SCIENCES-NANOSCIENCE AND NANOTECHNOLOGY
Volume 12, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2043-6262/abffc8
Keywords
cytotoxicity study; anticancer activity; Protein binding; Punica granatum; nanomedicine
Funding
- Maulana Azad National Fellowship, Govt. of India
- Department of Science & Technology and Biotechnology (DSTB), Govt. of West Bengal [756 (Sanc.)/ST/P/ST/9G-34/2013]
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This study demonstrated the formation of silver nanoparticles using pomegranate fruit extract and investigated their bio applications, including anticancer activity against triple negative breast carcinoma and MDA-MB-468 cells through apoptotic pathway.
In this work formation of silver nanoparticles using pomegranate fruit (Punica granatum) extract and its bio applications were studied. Gradual appearance of a peak at 422 nm during the reduction of AgNO3 by the fruit extract confirmed the formation of AgNPs. FTIR spectra indicated the details of formation mechanism. Images obtained from scanning electron microscopy (SEM) and transmittance electron microscopy (TEM) confirmed that the sample contains spherical AgNPs and XRD analysis showed its (FCC) crystal structure. This study also revealed that the synthesised AgNPs were monocrystalline in nature. Application of these AgNPs were studied measuring their affinity to bovine serum albumin (BSA) and human serum albumin (HSA). Cytotoxicity of the synthesised AgNPs was determined by DAPI staining. The results showed that the nanoparticles have significant anticancer activity against triple negative breast carcinoma and MDA-MB-468 cells. We concluded that the cell death was routed through apoptotic pathway.
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