Journal
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
Volume -, Issue -, Pages 366-369Publisher
IEEE
DOI: 10.1109/MEMS51782.2021.9375425
Keywords
Ex-tracellular vesicles; microRNA; digital polymerase chain reaction; ovarian cancer; point-of-care; microfluidics
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Funding
- Ministry of Science and Technology (MOST) of Taiwan [MOST 109-2813-C-007 -036 -E, MOST 1092221-E-007-006-MY3]
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This study used an automated method to detect microRNA-21 in plasma, achieving a very low limit of detection of 1.4 attomolar. The promising device could potentially be used for clinical cancer diagnosis and point-of-care applications in the near future.
In this study, one of microRNAs associated with ovarian cancer, microRNA-21, was extracted from extracellular vesicles in plasma, isolated by antibody-coated magnetic beads, and finally quantified by a digital polymerase chain reaction module on an integrated microfluidic platform, which integrated two functional modules, including a sample pretreatment and a microRNA quantification module. With this approach, microRNA-21 extraction and quantification could be automated and the limit of detection could be as low as only 1.4 attomolar. This promising device may be therefore utilized for clinical cancer diagnosis and point-of-care applications in the near future.
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