Journal
ANNALS OF DERMATOLOGY
Volume 33, Issue 4, Pages 339-344Publisher
KOREAN DERMATOLOGICAL ASSOC
DOI: 10.5021/ad.2021.33.4.339
Keywords
Differentiation; Keratinocytes; MDA5; Psoriasis
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Funding
- Chungnam National University Hospital Research Fund
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The study uncovered the possible roles of MDA5 in psoriasis. MDA5 expression was found to be increased in psoriatic skin and negatively regulated keratinocyte differentiation.
Background: Psoriasis is a chronic inflammatory skin disease. The etiology of psoriasis is not fully understood, but the genetic background is considered to be the most important factor. To date, many psoriasis-related genes have been discovered, but the role of many important genes has not been well understood. Objective: The purpose of this study is to uncover possible roles of MDA5 in psoriasis. Methods: Expression of MDA5 was investigated using immunohistochemistry. Then, MDA5 was overexpressed in keratinocytes using a recombinant adenovirus. Results: As a result of immunohistochemical staining, the expression of MDA5 was significantly increased in the epidermis of psoriasis compared to normal skin. Similarly, the expression of MDA5 was increased in imiquimod-induced psoriasiform dermatitis model. In cultured keratinocytes, toll-like receptor 3 agonist poly(I:C) induced expression of MDA5 at both mRNA and protein levels. When MDA5 was overexpressed using a recombinant adenovirus, poly(I:C)-induced cytokine expression was significantly increased. Finally, MDA5 overexpression significantly inhibited calcium-induced differentiation of keratinocytes. Conclusion: These results suggest that MDA5 increases in psoriasis and negatively regulates keratinocyte differentiation.
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