Journal
TREE PHYSIOLOGY
Volume 41, Issue 6, Pages 1077-1086Publisher
OXFORD UNIV PRESS
DOI: 10.1093/treephys/tpaa163
Keywords
fruit quality; Prunus persica; SDH activity; SnRK1 protein kinase; sorbitol; sugar metabolism
Categories
Funding
- National Nature Science Foundation of China [31672099]
- National Modern Agro-industry Technology Research System Fund [CARS-30-2-02]
- Natural Science Foundation of Shandong Province
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The study revealed that SnRK1 protein kinase responds to trehalose and sorbitol treatments in peach fruit, promoting sorbitol metabolism and enhancing sucrose accumulation. These findings suggest a potential for SnRK1 in improving fruit quality.
Fruit quality depends largely on the type and amount of sugar accumulated in the fruit. In peach [Prunus persica (L.) Batsch], sorbitol is the main photosynthetic product and plays a crucial role in sugar metabolism. As a conserved energy sensor, SNF1-related kinase 1 (SnRK1) is involved in the regulation of carbon metabolism. In this study, SnRK1 was able to respond to induction by treatment with exogenous trehalose and sorbitol on 'Ruipan 17' peach fruit. After treatment with 100-mM trehalose for 3 h, the SnRK1 activity decreased by 18% and the activities of sorbitol dehydrogenase (SDH) and sucrose synthase (SS) also decreased significantly, but sucrose phosphate synthase (SPS) activity increased significantly; whereas sorbitol treatment under the same conditions resulted in a 12.6% increase in SnRK1 activity and the activities of SDH and SS synthase also increased significantly, compared with the control. The contents of glucose, fructose and sucrose in peach fruit increased significantly after 3 h of sorbitol treatment. In addition, the interactions between PpSnRK1 alpha and enzymes PpSDH and PpSPS were confirmed by yeast two-hybrid method and the phosphorylation of PpSnRK1 alpha and PpSDH was detected in vitro. Taken together, these results suggest that SnRK1 promotes sorbitol metabolism by activating SDH and it also regulates the activities of SS and SPS that enhance sucrose accumulation in peach fruit. SnRK1 protein kinase is involved in sugar metabolism and has the potential to be used for improving fruit quality.
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