Journal
FRONTIERS IN FUNGAL BIOLOGY
Volume 2, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/ffunb.2021.692549
Keywords
high-throughput sequencing; aquatic plant; endophytic fungi; fresh water; fungal composition and diversity
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Funding
- National Natural Science Foundation Program of PR China [31770026, 31970013]
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This study investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent methods. The results revealed abundant endophytic fungal diversity in aquatic plants without differences in fungal isolation rates among tissues. Batrachium bungei was found to harbor the highest number of OTUs among the three most common plant species studied.
Aquatic ecosystems contain tremendous plant and microbial diversity. However, little is known about endophyte diversity in aquatic plants. In this study, we investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent high-throughput sequencing methods. A total of 1,689 fungal isolates belonging to three phyla and 154 genera were obtained from 15,373 plant tissue segments of 30 aquatic plant species. The most abundant endophytic fungi were those in ascomycete genera Aspergillus, Ceratophoma, Fusarium, Penicillium, Phoma and Plectosporium. No difference in fungal isolation rates was observed among tissues from roots, stems, and leaves. Twenty tissue samples from three most common plant species were further subjected to culture-independent meta-barcode sequencing. The sequence-based analyses revealed a total of 1,074 OTUs belonging to six fungal phyla and 194 genera. Among the three plants, Batrachium bungei harbored the highest number of OTUs. Besides, a total of 66 genera were detected by two methods. Both the culture-dependent and independent methods revealed that aquatic plants in southwest China have abundant endophytic fungal diversity. This study significantly expands our knowledge of the fungal community of aquatic plants.
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