Evaluation and Refinement of Sample Preparation Methods for Extracellular Matrix Proteome Coverage

The extracellular matrix is a key component of tissues, yet it is underrepresented in proteomic datasets. Identification and evaluation of proteins in the extracellular matrix (ECM) has proved challenging due to the insolubility of many ECM proteins in traditional protein extraction buffers. Here we separate the decellularization and ECM extraction steps of several prominent methods for evaluation under real-world conditions. The results are used to optimize a two-fraction ECM extraction method. Approximately one dozen additional parameters are tested, and recommendations for analysis based on overall ECM coverage or specific ECM classes are given. Compared with a standard in-solution digest, the optimized method yielded a fourfold improvement in unique ECM peptide identifications.

Automated summary

The study reveals the challenge of identifying proteins in the extracellular matrix but demonstrates a significant improvement through optimized extraction methods. Different approaches to ECM extraction were explored in the research, providing insights for better evaluation of ECM proteins.