Dopamine D1 receptor activation drives plasticity in the songbird auditory pallium

Vocal learning species must form and extensively hone associations between sounds and social contingencies. In songbirds, dopamine signaling guides song motor-production, variability, and motivation, but it is unclear how dopamine regulates fundamental auditory associations for learning new sounds. We hypothesized that dopamine regulates learning in the auditory pallium, in part by interacting with local neuroestradiol signaling. Here, we show that zebra finch auditory neurons frequently coexpress D1 receptor (D1R) protein, neuroestradiol-synthase, GABA, and parvalbumin. Auditory classical conditioning increased neuroplasticity gene induction in D1R-positive neurons. In vitro, D1R pharmacological activation reduced the amplitude of GABAergic and glutamatergic currents and increased the latter's frequency. In vivo, D1R activation reduced the firing of putative interneurons, increased the firing of putative excitatory neurons, and made both neuronal types unable to adapt to novel stimuli. Together, these findings support the hypothesis that dopamine acting via D1Rs modulates auditory association in the songbird sensory pallium.

Automated summary

The study demonstrates that dopamine interacts with local neuroestradiol signaling to modulate auditory learning in songbirds. D1 receptors (D1R) are frequently expressed in auditory neurons, and D1R activation reduces inhibitory activity while increasing excitatory firing rates.