4.2 Article

New Validated Diffuse Reflectance Infrared Fourier Transform Spectroscopic Method for the Quantification of Levofloxacin in Pharmaceutical Dosage Form

Journal

INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 83, Issue 3, Pages 430-436

Publisher

INDIAN PHARMACEUTICAL ASSOC
DOI: 10.36468/pharmaceutical-sciences.791

Keywords

Levofloxacin; fourier transform infrared spectroscopy; diffuse reflectance infrared fourier transform spectroscopy; validation; quantification; analysis

Funding

  1. RAKMHSU Research & Ethics Committee [RAKMHSU-REC-5-2016-PG-P]

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The validated diffuse reflectance infrared Fourier transform spectroscopic method for quantitative analysis of levofloxacin in solid dosage forms provides an eco-friendly approach with precise and accurate results. The linear calibration curve obtained in the study demonstrated high correlation coefficient and the method was found to be suitable for solid state samples. The proposed method showed good precision, accuracy and reproducibility, making it appropriate for quantitative analysis of levofloxacin in solid dosage forms.
Levofloxacin is a synthetic fluoroquinolone antibiotic available in different dosage forms for the treatment of various bacterial infections. Here in, we report the validated diffuse reflectance infrared Fourier transform spectroscopic method for quantitative analysis of levofloxacin in the solid dosage forms. This method offers the advantage of being eco-friendly that is applied for solid state samples. Appropriate quantities of levofloxacin and dry potassium bromide were mixed to get the desired concentration of samples for Fourier transform infrared spectroscopic analysis using a diffuse reflectance sampling interface. The peak in the Fourier transform infrared spectrum around 1724 cm(-1) was observed for the carbonyl group of levofloxacin. The calibration curve was plotted for the area under the curve of carbonyl peak against the concentration of calibration standards. A linear calibration curve with a correlation coefficient of 0.999 was obtained in the range of 0.3-1.8 % w/w of levofloxacin. The calibration curve method was linear, precise and accurate as per guidelines. The method employed for quantification of levofloxacin in three marketed tablet dosage forms and laboratory physical mixture yielded a mean recovery of 101.32 and 98.11 percent by weight, respectively. Overall, the precision, accuracy and reproducibility of the proposed diffuse reflectance infrared Fourier transform spectroscopy method make it appropriate for quantitative analysis of levofloxacin in solid dosage forms.

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