Journal
BIOMATERIALS SCIENCE
Volume 9, Issue 17, Pages 5854-5867Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1bm00570g
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Funding
- Japan Society for the Promotion of Science (JSPS)
- STINT
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan [26702017, 15KK0230, 18KK0305, 19K22951]
- Swedish Research Council [2018-04199, 2016-2075-5.1, 2016-04519]
- Eurostars-2 joint program [E! 113670]
- European Union
- German Federal Ministry of Education and Research
- RVO
- VINNOVA
- Grants-in-Aid for Scientific Research [26702017, 18KK0305, 15KK0230, 19K22951] Funding Source: KAKEN
- Swedish Research Council [2018-04199] Funding Source: Swedish Research Council
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The study demonstrates that PMPC-lipids can be used for liposomal coating, suppressing the adsorption of albumin, C3, and fibrinogen, and potentially serving as an alternative to PEG.
Poly(ethylene glycol) (PEG) is frequently used for liposomal surface modification. However, as PEGylated liposomes are cleared rapidly from circulation upon repeated injections, substitutes of PEG are being sought. We focused on a water-soluble polymer composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units, and synthesized poly(MPC) (PMPC)-conjugated lipid (PMPC-lipid) with degrees of MPC polymerization ranging from 10 to 100 (calculated molecular weight: 3 to 30 kDa). In addition, lipids with three different alkyl chains, myristoyl, palmitoyl, and stearoyl, were applied for liposomal surface coating. We studied the interactions of PMPC-lipids with plasma albumin, human complement protein C3 and fibrinogen using a quartz crystal microbalance with energy dissipation, and found that adsorption of albumin, C3 and fibrinogen could be suppressed by coating with PMPC-lipids. In particular, the effect was more pronounced for PMPC chains with higher molecular weight. We evaluated the size, polydispersity index, surface charge, and membrane fluidity of the PMPC-lipid-modified liposomes. We found that the effect of the coating on the dispersion stability was maintained over a long period (98 days). Furthermore, we also demonstrated that the anti-PEG antibody did not interact with PMPC-lipids. Thus, our findings suggest that PMPC-lipids can be used for liposomal coating.
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