Journal
ANIMAL SCIENCE JOURNAL
Volume 92, Issue 1, Pages -Publisher
WILEY
DOI: 10.1111/asj.13597
Keywords
berberine; chicken; myoblast; myogenetic oligodeoxynucleotide (myoDN); myogenic differentiation
Categories
Funding
- Nagano Prefecture to Promote Scientific Activity [H29-3-12]
- Ito Foundation
- Japan Society for the Promotion of Science [18K05939, 19J20888, 19K05948]
- Kieikai Research Foundation [2018C002]
- Shinshu Foundation for Promotion of Agricultural and Forest Science
- Grants-in-Aid for Scientific Research [19J20888, 19K05948, 18K05939] Funding Source: KAKEN
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Research has shown that certain myogenetic oligodeoxynucleotides (myoDNs) can promote the differentiation of chicken myoblasts into MHC-positive myotubes.
Myoblasts are myogenic precursors that develop into myotubes during muscle formation. Improving efficiency of myoblast differentiation is important for advancing meat production by domestic animals. We recently identified novel oligodeoxynucleotides (ODNs) termed myogenetic ODNs (myoDNs) that promote the differentiation of mammalian myoblasts. An isoquinoline alkaloid, berberine, forms a complex with one of the myoDNs, iSN04, and enhances its activities. This study investigated the effects of myoDNs on chicken myoblasts to elucidate their species-specific actions. Seven myoDNs (iSN01-iSN07) were found to facilitate the differentiation of chicken myoblasts into myosin heavy chain (MHC)-positive myotubes. The iSN04-berberine complex exhibited a higher myogenetic activity than iSN04 alone, which was shown to enhance the differentiation of myoblasts into myotubes and the upregulation of myogenic gene expression (MyoD, myogenin, MHC, and myomaker). These data indicate that myoDNs promoting chicken myoblast differentiation may be used as potential feed additives in broiler diets.
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