Journal
MATERIALS CHEMISTRY FRONTIERS
Volume 5, Issue 18, Pages 6850-6859Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1qm00296a
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Funding
- Thailand Research Fund [RSA6080012]
- National Research Council of Thailand (NRCT) [NRCT5-RSA63001-23]
- Durham University, UK
- Chulalongkorn University, Thailand
- Development and Promotion of Science and Technology Talent Projects (DPST)
- UK Engineering and Physical Sciences Research Council
- Soft Matter and Functional Interfaces Centre for Doctoral Training
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A novel fluorescent hybrid material was developed for detecting organophosphates with high sensitivity and a wide linear range. This graphene quantum dots and enzyme supported supramolecular hydrogel shows promising potential for the detection of gaseous organophosphate pesticides.
Developing the sensitivity of an optical biosensor motivates the exploration of an amplification strategy for the incorporation of a biosensor system in a supramolecular hydrogel. We developed novel fluorescent hybrid materials comprising graphene quantum dots and enzymes supported by L-phenylalanine derived bis-(urea) supramolecular hydrogels (GQDs/Enz/Gels) for detection of organophosphates. The addition of acetylcholinesterase (AChE) and choline oxidase (ChOx) during the formation of self-assembled GQDs/Gel materials resulted in enzyme-functionalized gel networks. Significant turn-off fluorescence of the encapsulated GQDs in the hydrogels was due to hydrogen peroxide generated from the active enzymatic reaction. The addition of insecticide dichlorvos to the GQDs/Enz/Gels resulted in the recovery of fluorescence in proportion to the concentration of dichlorvos, with a detection limit of 2.61 x 10(-8) M which outperforms those in solution by 100-fold improvement and a wider linear range of 1.25 x 10(-8)-1.25 x 10(-4) M compared to those of the biosensor in solution. These hybrid hydrogels show promising sensitivity to the detection of oxy-form organophosphate pesticides and expectedly offer scope for the development of rapid and environmentally friendly techniques.
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