Journal
ANALYST
Volume 146, Issue 16, Pages 5204-5211Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1an00980j
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21976105]
- Natural Science Foundation of Shandong Province [ZR2020QB149]
Ask authors/readers for more resources
In this study, a reliable near-infrared ratiometric fluorescent biosensor CDMS was designed and constructed for real-time monitoring of ONOO- fluctuations in tumor progression. The probe demonstrated excellent performance in cellular imaging and in vivo visualization of ONOO- in tumor pathological progression, showing great potential for tumor diagnosis and therapy.
Malignant tumors are one of the main causes for human death and are tightly associated with overexpression of reactive oxygen species (ROS) in pathological processes. Therefore, in vivo monitoring of ROS, especially ONOO-, remains of great significance for diagnosis and therapy of tumors to improve the survival rate. Herein, we designed and constructed a reliable near-infrared (NIR) ratiometric fluorescent biosensor CDMS for monitoring the fluctuations of ONOO- in the process of tumor progression. CDMS featured outstanding stability to photoirradiation, substantial quantum yields, rapid response (<5 s), high selectivity and excellent biocompatibility. Moreover, CDMS exhibited distinct ratiometric fluorescence signal changes after reacting with ONOO-. Fluorescence imaging in immune stimulated cells indicated that CDMS was competent to determine the levels of ONOO- in the cellular level. Remarkably, CDMS was further applied in monitoring the expression of ONOO- in a peritonitis mouse model and tumor-bearing mouse model. Based on the excellent properties of CDMS, the probe exhibited the potential for noninvasive in vivo visualization of ONOO- in the occurrence and process of tumor development. It is envisioned that CDMS can be employed as a promising tool for monitoring the ONOO- fluxes in tumor pathological progression, especially for tumor diagnosis and therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available