Journal
THERANOSTICS
Volume 11, Issue 17, Pages 8480-8499Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.60031
Keywords
optic neuritis; astrocytes; YAP; TGF-beta 1; neuroinflammation
Categories
Funding
- Natural Science Foundation of Zhejiang Province [LR18C090001, LR21H090001]
- National Natural Science Foundation [92049104, 81971142, 81971172]
- Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions [NYKFKT2019008]
- Hangzhou Normal University [4125C5021920453]
- Public Welfare Science and Technology Project of Wenzhou [Y20190132]
- Zhejiang Province Science and Technology Plan Research and Xinmiao Talent Program [2019R413078, 2020R413077]
- National College Students Innovation and Entrepreneurship Training Program [20201034039]
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The study showed that YAP was significantly upregulated and activated in astrocytes of optic nerve in EAE mice. Conditional knockout of YAP in astrocytes resulted in more severe inflammatory infiltration and demyelination in the optic nerve, as well as promoted activation of astrocytes and microglia while inhibiting astrocyte proliferation. The downregulation of the TGF-beta signaling pathway after YAP deletion in astrocytes was also observed, with potential therapeutic agents like SRI-011381 and XMU-MP-1 showing promising effects in rescuing optic nerve deficits and relieving neuroinflammation in EAE mice.
Rationale: Optic neuritis is one of main symptoms in multiple sclerosis (MS) that causes visual disability. Astrocytes are pivotal regulators of neuroinflammation in MS, and astrocytic yes-associated protein (YAP) plays a critical role in neuroinflammation. Meanwhile, YAP signaling is involved in visual impairment, including glaucoma, retinal choroidal atrophy and retinal detachment. However, the roles and underlying mechanisms of astrocytic YAP in neuroinflammation and demyelination of MS-related optic neuritis (MS-ON) remains unclear. Methods: To assess the functions of YAP in MS-ON, experimental autoimmune encephalomyelitis (EAE, a common model of MS) was established, and mice that conditional knockout (CKO) of YAP in astrocytes, YAP(GFAP)-CKO mice, were successfully generated. Behavior tests, immunostaining, Nissl staining, Hematoxylin-Eosin (HE) staining, TUNEL staining, Luxol Fast Blue (LFB) staining, electron microscopy (EM), quantitative real-time PCR (qPCR), gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) by RNA sequencing were used to examine the function and mechanism of YAP signaling based on these YAP(GFAP)-CKO mice and EAE model mice. To further explore the potential treatment of YAP signaling in EAE, EAE mice were treated with various drugs, including SRI-011381 that is an agonist of transforming growth factor-beta (TGF-beta) pathway, and XMU-MP-1 which inhibits Hippo kinase MST1/2 to activate YAP. Results: We found that YAP was significantly upregulated and activated in the astrocytes of optic nerve in EAE mice. Conditional knockout of YAP in astrocytes caused more severe inflammatory infiltration and demyelination in optic nerve, and damage of retinal ganglion cells (RGCs) in EAE mice. Moreover, YAP deletion in astrocytes promoted the activation of astrocytes and microglia, but inhibited the proliferation of astrocytes of optic nerve in EAE mice. Mechanically, TGF-beta signaling pathway was significantly down-regulated after YAP deletion in astrocytes. Additionally, both qPCR and immunofluorescence assays confirmed the reduction of TGF-beta signaling pathway in YAP(GFAP)-CKO EAE mice. Interestingly, SRI-011381 partially rescued the deficits in optic nerve and retina of YAP(GFAP)-CKO EAE mice. Finally, activation of YAP signaling by XMU-MP-1 relieved the neuroinflammation and demyelination in optic nerve of EAE mice. Conclusions: These results suggest astrocytic YAP may prevent the neuroinflammatory infiltration and demyelination through upregulation of TGF-beta signaling and provide targets for the development of therapeutic strategies tailored for MS-ON.
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