4.7 Article

IgE epitope analysis of sarcoplasmic-calcium-binding protein, a heat-resistant allergen in Crassostrea angulata

Journal

FOOD & FUNCTION
Volume 12, Issue 18, Pages 8570-8582

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/d1fo01058a

Keywords

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Funding

  1. National Natural Scientific Foundation of China [32072336, 31871720, 32001695, 31901811]
  2. Science and Technology Program of Fujian Province [2018N5009, 2018R0071]
  3. Science and Technology Program of Xiamen City [3502Z20203059]
  4. National Key RAMP
  5. D Program of China [2019YFD0901703]
  6. Marine Scientific Research Special Foundation for Public Sector Program [DY135-B2-07]

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SCP has been identified as a novel allergen in Crassostrea angulata, inducing basophil activation and showing significant allergenicity. The study successfully analyzed five linear epitopes and two conformational epitopes of SCP, as well as observed changes in its structural characteristics and IgE-binding capacity after Ca2+ depletion. These epitope data enhance the understanding of oyster allergens and could potentially lead to the development of hypoallergenic shellfish products.
Sarcoplasmic-calcium-binding protein (SCP) has been investigated as a novel allergen in Crassostrea angulata. Nevertheless, knowledge of its effector-cell-based allergic relevance and epitopes is limited. In this study, the heat-resistant allergen SCP was able to induce significant upregulation of CD63 and CD203c (p < 0.05), which showed obvious allergenicity in a basophil activation test. Furthermore, immunoinformatic tools, a one-bead-one-compound peptide library, and phage display technology were combined to analyze the allergenic epitopes of SCP. Five linear epitopes named L-SCP-1 (AA(22-33)), L-SCP-2 (AA(64-75)), L-SCP-3 (AA(80-90)), L-SCP-4 (AA(107-116)), and L-SCP-5 (AA(144-159)) were verified using serological tests. Additionally, two conformational epitopes (C-SCP-1 and C-SCP-2) were determined, and C-SCP-1 was located at one of the calcium-binding sites (AA(106-117)). Moreover, SCP showed weaker typical alpha-helical features and higher hydrophobicity after Ca2+ depletion, which reduced its IgE-binding capacity. Overall, these epitope data could enhance our understanding of oyster allergens, which could be used to develop hypoallergenic shellfish products.

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