Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 9, Issue 35, Pages 7145-7166Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1tb01430g
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Funding
- Australian Government Research Scholarship
- University of Queensland
- National Health and Medical Research Council [GNT1071796, GNT1146627]
- National Health and Medical Research Council (NHMRC) [GNT1143296]
- Australian Research Council (ARC) [DP200102723]
- Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy and Microanalysis
- Australian Research Council [DP200102723] Funding Source: Australian Research Council
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The development of silica nanoparticles-based formulations enhances the epithelial permeability of Vancomycin. Silica nanoparticles with large pores and functionalized with -CH3 groups show prolonged release and permeation enhancing effect. This study highlights the potential of silica nanoparticles, especially those with large pores, for oral delivery of Vancomycin or other antimicrobial peptides.
Vancomycin (Van) is a key antibiotic of choice for the treatment of systemic methicillin resistant Staphylococcus aureus (MRSA) infections. However, due to its poor membrane permeability, it is administered parenterally, adding to the cost and effort of treatment. The poor oral bioavailability of Van is mainly due to its physico-chemical properties that limit its paracellular and transcellular transport across gastrointestinal (GI) epithelium. Herein we report the development of silica nanoparticles (SNPs)-based formulations that are able to enhance the epithelial permeability of Van. We synthesized SNPs of different pore sizes (2 nm and 9 nm) and modified their surface charge and polarity by attaching different functional groups (-NH2, -PO3, and -CH3). Van was loaded within these SNPs at a loading capacity in the range of ca. 18-29 wt%. The Van-loaded SNPs exhibited a controlled release behaviour when compared to un-encapsulated Van which showed rapid release due to its hydrophilic nature. Among Van-loaded SNPs, SNPs with large pores showed a prolonged release compared to SNPs with small pores while SNPs functionalised with -CH3 groups exhibited a slowest release among the functionalised SNPs. Importantly, Van-loaded SNPs, especially the large pore SNPs with negative charge, enhanced the permeability of Van across an epithelial cell monolayer (Caco-2 cell model) by up to 6-fold, with P-app values up to 1.716 x 10(-5) cm s(-1) (vs. 0.304 x 10(-5) cm s(-1) for un-encapsulated Van) after 3 h. The enhancement was dependent on both the type of SNPs and their surface functionalisation. The permeation enhancing effect of SNPs was due to its ability to transiently open the tight junctions measured by decrease in transepithelial resistance (TEER) which was reversible after 3 h. All in all, our data highlights the potential of SNPs (especially SNPs with large pores) for oral delivery of Van or other antimicrobial peptides.
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