Exploring the use of upconversion nanoparticles in chemical and biological sensors: from surface modifications to point-of-care devices

Upconversion nanoparticles (UCNPs) have emerged as promising luminescent nanomaterials due to their unique features that allow the overcoming of several problems associated with conventional fluorescent probes. Although UCNPs have been used in a broad range of applications, it is probably in the field of sensing where they best evidence their potential. UCNP-based sensors have been designed with high sensitivity and selectivity, for detection and quantification of multiple analytes ranging from metal ions to biomolecules. In this review, we deeply explore the use of UCNPs in sensing systems emphasizing the most relevant and recent studies on the topic and explaining how these platforms are constructed. Before diving into UCNP-based sensing platforms it is important to understand the unique characteristics of these nanoparticles, why they are attracting so much attention, and the most significant interactions occurring between UCNPs and additional probes. These points are covered over the first two sections of the article and then we explore the types of fluorescent responses, the possible analytes, and the UCNPs' integration with various material types such as gold nanostructures, quantum dots and dyes. All the topics are supported by analysis of recently reported sensors, focusing on how they are built, the materials' interactions, the involved synthesis and functionalization mechanisms, and the conjugation strategies. Finally, we explore the use of UCNPs in paper-based sensors and how these platforms are paving the way for the development of new point-of-care devices.

Automated summary

Upconversion nanoparticles (UCNPs) have emerged as promising luminescent nanomaterials with unique features that allow them to overcome problems associated with conventional fluorescent probes. Particularly in the field of sensing, UCNP-based sensors have shown high sensitivity and selectivity for detecting and quantifying a wide range of analytes. This review explores the use of UCNPs in sensing systems, emphasizing recent studies and explaining how these platforms are constructed, as well as discussing their unique characteristics and interactions with additional probes.