High Performance Liquid Chromatography-Tandem Quadrupole-Time of Flight-Mass Spectrometry for Determination of Indaziflam and Four Metabolites in Cereals

A high performance liquid chromatography-tandem quadrupole-time of flight-mass spectrometry ( HPLC-Q-TOF/MS ) method for determination of residues of indaziflam and its four metabolites (1-fluoroethyl triazinediamine , indaziflam-carboxylic acid , triazine-indanone and indaziflam-olefin) in cereal samples was developed. The samples were extracted by acetonitrile , and the supernatant was collected for purification after salting-out and centrifugation. Under the optimized chromatographic and mass spectrometric conditions , the data were collected by electrospray ionization ( ESI) sources in positive ion mode. The calibration curves of five compounds showed good linearity in concentration range of 2. 0 -200 mu g/L ( R-2 >0. 9987) . The average recoveries were 79. 3% -108. 0% at three spiked levels ( 10 , 20 and 100 mu g/kg) , while the relative standard deviations ( RSD) were 1. 2% -6. 0%. The limits of detection and limits of quantification ranged from 0. 05 to 0. 60 mu g/kg and 0. 15 to 2. 00 mu g/kg, respectively. This method was a simple and efficient method with high sensitivity and precision , and had practical value for the rapid detection of residues of indaziflam and its four metabolites in cereals.

Automated summary

A HPLC-Q-TOF/MS method was developed for rapid detection of residues of indaziflam and its metabolites in cereals, showing high sensitivity and precision with practical value for detection work.