Potential role of anti-inflammatory HDL subclasses in metabolic unhealth/obesity

High-density lipoprotein (HDL) particles comprising heterogeneous subclasses of different functions exert anti-inflammatory effects by interacting with immune-response cells. However, the relationship of HDL subclasses with immune-response cells in metabolic unhealth/obesity has not been defined clearly. The purpose of this study was to delineate the relational changes of HDL subclasses with immune cells and inflammatory markers in metabolic unhealth/obesity to understand the role of anti-inflammatory HDL subclasses. A total of 316 participants were classified by metabolic health. HDL subclasses were detected by microfluidic chip electrophoresis. White blood cell (WBC) counts and lymphocytes were assessed using automatic haematology analyser. Levels of high-sensitivity C-reactive protein (hs-CRP) and interleukin 6 (IL-6) were measured. In our study, not only the distribution of HDL subclasses, but also HDL-related structural proteins changed with the deterioration of metabolic disease. Moreover, lymphocytes and inflammation factors significantly gradually increased. The level of HDL2b was negatively associated with WBC, lymphocytes and hs-CRP in multivariable linear regression analysis. In multinomial logistic regression analysis, high levels of HDL3 and low levels of HDL2b increased the probability of having an unfavourable metabolic unhealth/obesity status. We supposed that HDL2b particles may play anti-inflammation by negatively regulating lymphocytes activation. HDL2b may be a therapeutic target for future metabolic disease due to the anti-inflammatory effects.

Automated summary

The study found that with the deterioration of metabolic disease, the distribution of HDL subclasses and related structural proteins also changed, while lymphocytes and inflammation factors gradually increased. The level of HDL2b was negatively associated with WBC, lymphocytes, and hs-CRP, and high levels of HDL3 and low levels of HDL2b increased the probability of having an unfavorable metabolic unhealth/obesity status. This suggests that HDL2b particles may play an anti-inflammatory role by negatively regulating lymphocyte activation and could be a potential therapeutic target for future metabolic diseases.