De novo genome assembly of the tobacco hornworm moth (Manduca sexta)

The tobacco hornworm, Manduca sexta, is a lepidopteran insect that is used extensively as a model system for studying insect biology, development, neuroscience, and immunity. However, current studies rely on the highly fragmented reference genome Msex_1.0, which was created using now-outdated technologies and is hindered by a variety of deficiencies and inaccuracies. We present a new reference genome for M. sexta, JHU_Msex_v1.0, applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. The assembly is 470Mb and is similar to 20x more continuous than the original assembly, with scaffold N50> 14Mb. We annotated the assembly by lifting over existing annotations and supplementing with additional supporting RNA-based data for a total of 25,256 genes. The new reference assembly is accessible in annotated form for public use. We demonstrate that improved continuity of the M. sexta genome improves resequencing studies and benefits future research on M. sexta as a model organism.

Automated summary

Researchers introduced a new, more complete reference genome JHU_Msex_v1.0 for the tobacco hornworm, generated using modern technologies to improve genome continuity and accuracy. The assembly includes 25,256 genes and is accessible for public use, demonstrating benefits for future research on the tobacco hornworm as a model organism.