Journal
EUROPEAN JOURNAL OF PROTISTOLOGY
Volume 61, Issue -, Pages 265-277Publisher
ELSEVIER GMBH
DOI: 10.1016/j.ejop.2017.06.007
Keywords
Astomatida; Ciliophora; Cirratulidae; Endocytoplasmic bacteria; Microscopy; Molecular phylogeny
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Funding
- ANR HAPAR
- Natural Sciences and Engineering Research Council of Canada Discovery Grant [F12-04977]
- Ministere de la Recherche
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Microscopic and phylogenetic analyses were performed on endocommensal astome ciliates retrieved from the middle intestine of a marine cirratulid polychaete, Cirriformia tentaculata, collected in the bay of Roscoff (English Channel, Northwest French coast) and on the Southwest English coast. Three morphotypes of the astome genus Durchoniella were identified, two corresponding to described species (the type species Durchoniella brasili (Leger and Duboscq, 1904) De Puytorac, 1954 and Durchoniella legeriduboscqui De Puytorac, 1954) while a third morphotype remains undescribed. Their small subunit (SSU) rRNA gene sequences showed at least 97.2% identity and phylogenetic analyses grouped them at the base of the subclass Scuticociliatia (Oligohymenophorea), as a sister lineage to all astomes from terrestrial oligochaete annelids. Ultrastructural examination by transmission electron microscopy and fluorescence in situ hybridization analyses revealed the presence of endocytoplasmic cocci and rod-shaped bacteria surrounded by a very thin membrane. These endocytoplasmic bacteria may play a role in the association between endocommensal astome ciliates and cirratulid polychaetes inhabiting in anoxic coastal sediments. (C) 2017 Elsevier GmbH. All rights reserved.
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