Journal
FOOD & FUNCTION
Volume 12, Issue 19, Pages 9327-9338Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1fo01683k
Keywords
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Funding
- National Natural Science Foundation of China [31700015]
- Anhui Natural Science Foundation [1808085QC66]
- Development and Reform Commission of Hunan Province [412]
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The chemical structure and immunomodulatory ability of extracellular polysaccharide MEP 2a from Morchella esculenta were investigated, providing a basis for further research and potential applications.
The extracellular polysaccharide of Morchella esculenta cultivated under submerged fermentation was extracted. A single polysaccharide was purified through DEAE-Cellulose 52 and Sephadex G 100, and named as MEP 2a. The molecular weight of MEP 2a was determined by HPGPC and it is about 1391.5 kDa. MEP 2a is composed of mannose and glucose as the monosaccharide unit with a molar ratio of 8.15 : 1.07. The main polysaccharide chemical structure was analyzed by 1D and 2D NMR. Methylation and NMR analysis revealed that the backbone of MEP 2a consists of 1,3,4-linked-Manp, 1,2-linked-Manp and 1,6-linked-Glcp. 1D and 2D NMR results indicated that the main chain is based on -> 1)-beta-d-Glcp-(6 ->, -> 1)-alpha-d-Manp-(3,4 ->, -> 1)-alpha-d-Manp-(2 ->) and the branch chain is composed of alpha-d-Manp-(1 ->, -> 1)-beta-d-Glcp-(6 -> and alpha-d-Glcp-(1 ->). MEP 2a promoted the phagocytosis function and secretion of NO, IL-1 beta, IL-6 and TNF-alpha of macrophages. In the present study, the chemical structure and immunomodulatory ability of an extracellular polysaccharide of Morchella esculenta was investigated which guarantees further research studies and promising applications.
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