Squaric acid as a new chemoselective moiety for mass spectrometry-based metabolomics analysis of amines

The investigation of microbiome-derived metabolites is important to understand metabolic interactions with their human host. New methodologies for mass spectrometric discovery of undetected metabolites with unknown bioactivity are required. Herein, we introduce squaric acid as a new chemoselective moiety for amine metabolite analysis in human fecal samples.

Automated summary

Investigating microbiome-derived metabolites is crucial for understanding their metabolic interactions with the human host, and there is a need for new methodologies to discover metabolites with unknown bioactivity. In this study, squaric acid is introduced as a new chemoselective moiety for analyzing amine metabolites in human fecal samples.