Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 296, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1074/jbc.RA120.015402
Keywords
-
Categories
Funding
- Medical Scientist Training Program (MSTP) [T32GM007267]
- Cell and Molecular Biology (CMB) from NIH [T32GM008136]
- CMB training grant
- NIH [R21AG053596, RO1GM075240, R56AG059590, RO1AG043076, RO1AG045351, RO1CA214718, P30AG028716]
- David and Lucile Packard Foundation [2009-34710]
Ask authors/readers for more resources
Caloric restriction has been shown to improve the health span and life span of various organisms by influencing the metabolism of specific amino acids, such as L-serine, through the one-carbon metabolism pathway. This mechanism suggests a potential non-cell-autonomous regulation of life span, providing insights into interventions for aging-associated diseases.
Caloric restriction (CR) improves health span and life span of organisms ranging from yeast to mammals. Understanding the mechanisms involved will uncover future interventions for aging-associated diseases. In budding yeast, Saccharomyces cerevisiae, CR is commonly defined by reduced glucose in the growth medium, which extends both replicative and chronological life span (CLS). We found that conditioned media collected from stationary-phase CR cultures extended CLS when supplemented into nonrestricted (NR) cultures, suggesting a potential cell-nonautonomous mechanism of CR-induced life span regulation. Chromatography and untargeted metabolomics of the conditioned media, as well as transcriptional responses associated with the longevity effect, pointed to specific amino acids enriched in the CR conditioned media (CRCM) as functional molecules, with L-serine being a particularly strong candidate. Indeed, supplementing L-serine into NR cultures extended CLS through a mechanism dependent on the one-carbon metabolism pathway, thus implicating this conserved and central metabolic hub in life span regulation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available