Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 296, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jbc.2021.100585
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Funding
- National Nature and Science Foundation of China [32090052, 31570932, U1801286, 82071807, 82004046]
- Guangdong Provincial Key Laboratory of Regional Immunity and Diseases [2019B030301009]
- Medical and Health Technology Project of Guangzhou [20171A010042]
- Shenzhen Nanshan District Oversea Research Personnel Initiative Group Fund [LHTD20180007]
- Shenzhen science, technology and innovation committee [KQTD20170331145453160, GJHZ20180418190535757, KQJSCX20180328095619081]
- Shenzhen Key Medical Discipline Construction Fund [SZXK039]
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House dust mite-derived protein PDI binds to dendritic cells (DCs) and promotes Foxp3 expression through DEC205-mediated endocytosis, inducing the development of tolerogenic DCs. This pathway activation contributes to immune regulation and shows potential for treating immune disorders like asthma.
House dust mites (HDMs) are a potent allergen source that are commonly found in human living environments. While HDMs are known to induce allergic diseases in humans, such as asthma, its other biological activities related to human health are less understood. Our laboratory recently purified the HDM protein PDI (protein disulfide isomerase). In this study, we assess the role of PDI in contributing to immune regulation. Using mass spectrometry, we analyzed the complexes of DEC205 and HDM extracts, and the role of PDI in the induction of tolerogenic dendritic cells (DCs) was assessed in human cell culture experiments and verified in a murine model. We found that more than 20 HDM-derived proteins, including PDI, bound to DCs by forming complexes with DEC205. Additionally, DEC205-mediated the endocytosis of PDI. HDM-derived PDI (HDM-PDI) promoted Foxp3 expression in DCs. HDM-PDI-primed DCs also showed tolerogenic properties that induced regulatory T cell development, indicating that the primed DCs were tolerogenic DCs. Our results suggested that the PDI/DEC205/TIEG1/Foxp3 signal pathway activation was involved in the HDM-PDI-induced Foxp3 expression in DCs. Finally, we found that HDM-PDI competitively counteracted the Th2 cytokines to restore DC's tolerogenicity, and administration of HDM-PDI could suppress experimental asthma. In conclusion, our data suggest that HDM-PDI contributes to immune regulation by inducing tolerogenic DC development. Administration of HDM-PDI can alleviate experimental asthma. These findings demonstrate that HDM-PDI has translational potential to be used in the treatment of immune disorders such as asthma.
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