4.8 Article

An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts

Related references

Note: Only part of the references are listed.
Review Biochemistry & Molecular Biology

Cell-Free Exploration of the Natural Product Chemical Space

Jonathan W. Bogart et al.

Summary: Natural products and secondary metabolites are essential resources that remain largely undiscovered, but high-throughput DNA sequencing and computational analysis are enabling the discovery of new chemical scaffolds and enzymes through cell-free metabolic engineering. These enzymes can serve as flexible biocatalytic tools to expand the chemical space of natural products and provide a more sustainable manufacturing route.

CHEMBIOCHEM (2021)

Article Biochemical Research Methods

A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology

Simon J. Moore et al.

Summary: TX-TL systems show promise in studying natural product biosynthetic pathways with reduced experimental time and controlled conditions. Developing specialized cell-free systems is crucial, and the updated Streptomyces TX-TL protocol improves protein yield, demonstrating combined transcription, translation, and biosynthesis of Streptomyces metabolic pathways in a single reaction pot.

ACS SYNTHETIC BIOLOGY (2021)

Review Biochemical Research Methods

Toward sustainable, cell-free biomanufacturing

Blake J. Rasor et al.

Summary: Industrial biotechnology is an attractive approach for producing low-cost fuels and products from sustainable resources, but cells pose limitations on product synthesis and release. Cell-free biotechnology, utilizing biological machinery harvested from cells, offers advantages such as shorter development times, higher production rates, and tolerance to toxic molecules. Expanding the practice of cell-free biomanufacturing could lead to innovative approaches for producing green chemicals on an industrial scale.

CURRENT OPINION IN BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

A lysate proteome engineering strategy for enhancing cell-free metabolite production

David C. Garcia et al.

Summary: Cell-free systems provide an opportunity to utilize the metabolic potential of various organisms and explore novel chemical transformation systems without the need to maintain cell viability. While crude extracts retain much of a cell's capabilities, there is a lack of tools for engineering their contents in cell-free systems. By using MAGE to selectively deplete proteins from crude extracts, specific edits to central metabolism can enhance chemical production without impacting cell growth. This approach demonstrates the effectiveness of cell-free metabolic engineering for applications such as bioprototyping and bioproduction by combining systems and synthetic biology tools.

METABOLIC ENGINEERING COMMUNICATIONS (2021)

Review Genetics & Heredity

Cell-free gene expression: an expanded repertoire of applications

Adam D. Silverman et al.

NATURE REVIEWS GENETICS (2020)

Review Biotechnology & Applied Microbiology

Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production

James U. Bowie et al.

TRENDS IN BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

In vitro prototyping of limonene biosynthesis using cell-free protein synthesis

Quentin M. Dudley et al.

METABOLIC ENGINEERING (2020)

Review Biotechnology & Applied Microbiology

Biological Materials: The Next Frontier for Cell-Free Synthetic Biology

Richard J. R. Kelwick et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

Establishing a EukaryoticPichia pastorisCell-Free Protein Synthesis System

Lingkai Zhang et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Review Biotechnology & Applied Microbiology

Synthetic biology, systems biology, and metabolic engineering ofYarrowia lipolyticatoward a sustainable biorefinery platform

Jingbo Ma et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin

Lei Zhuang et al.

METABOLIC ENGINEERING (2020)

Article Biochemistry & Molecular Biology

In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design

Ashty S. Karim et al.

NATURE CHEMICAL BIOLOGY (2020)

Article Multidisciplinary Sciences

Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits

Luis E. Contreras-Llano et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

Michael E. Pyne et al.

NATURE COMMUNICATIONS (2020)

Review Biotechnology & Applied Microbiology

Cell-free systems for accelerating glycoprotein expression and biomanufacturing

Jasmine Hershewe et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

Cell-free styrene biosynthesis at high titers

William S. Grubbe et al.

METABOLIC ENGINEERING (2020)

Review Biotechnology & Applied Microbiology

Application of different types of CRISPR/Cas-based systems in bacteria

Zhenquan Liu et al.

MICROBIAL CELL FACTORIES (2020)

Article Multidisciplinary Sciences

Biosynthesis of medicinal tropane alkaloids in yeast

Prashanth Srinivasan et al.

NATURE (2020)

Article Multidisciplinary Sciences

Isobutanol production freed from biological limits using synthetic biochemistry

Saken Sherkhanov et al.

NATURE COMMUNICATIONS (2020)

Article Biotechnology & Applied Microbiology

Simple, functional, inexpensive cell extract for in vitro prototyping of proteins with disulfide bonds

Jared L. Dopp et al.

BIOCHEMICAL ENGINEERING JOURNAL (2020)

Article Biochemical Research Methods

Enhancing control of cell-free metabolism through pH modulation

Ashty S. Karim et al.

SYNTHETIC BIOLOGY (2020)

Review Biotechnology & Applied Microbiology

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Stephanie D. Cole et al.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2020)

Article Biotechnology & Applied Microbiology

A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds

Jennifer E. Kay et al.

METABOLIC ENGINEERING COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

A cell-free platform for the prenylation of natural products and application to cannabinoid production

Meaghan A. Valliere et al.

NATURE COMMUNICATIONS (2019)

Review Biochemical Research Methods

Recent advancements in fungal-derived fuel and chemical production and commercialization

Holly Ekas et al.

CURRENT OPINION IN BIOTECHNOLOGY (2019)

Article Biochemistry & Molecular Biology

Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems

Sung Sun Yim et al.

MOLECULAR SYSTEMS BIOLOGY (2019)

Article Biotechnology & Applied Microbiology

Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform

Rochelle Aw et al.

BIOTECHNOLOGY AND BIOENGINEERING (2019)

Review Biochemical Research Methods

A critical comparison of cellular and cell-free bioproduction systems

Nico J. Claassens et al.

CURRENT OPINION IN BIOTECHNOLOGY (2019)

Review Biochemical Research Methods

A User's Guide to Cell-Free Protein Synthesis

Nicole E. Gregorio et al.

METHODS AND PROTOCOLS (2019)

Review Biochemical Research Methods

Cell-Free Metabolic Engineering: Recent Developments and Future Prospects

Hye Jin Lim et al.

METHODS AND PROTOCOLS (2019)

Article Biochemical Research Methods

Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates

Quentin M. Dudley et al.

SYNTHETIC BIOLOGY (2019)

Review Biotechnology & Applied Microbiology

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanova et al.

BIOTECHNOLOGY ADVANCES (2018)

Review Biotechnology & Applied Microbiology

Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications

Jiazhang Lian et al.

METABOLIC ENGINEERING (2018)

Article Biochemistry & Molecular Biology

Adaptation of S. cerevisiae to Fermented Food Environments Reveals Remarkable Genome Plasticity and the Footprints of Domestication

Jean-Luc Legras et al.

MOLECULAR BIOLOGY AND EVOLUTION (2018)

Review Microbiology

Metabolic Engineering of Yeast for the Production of 3-Hydroxypropionic Acid

Rong-Yu Ji et al.

FRONTIERS IN MICROBIOLOGY (2018)

Review Biotechnology & Applied Microbiology

Expanding biological applications using cell-free metabolic engineering: An overview

James R. Swartz

METABOLIC ENGINEERING (2018)

Review Biotechnology & Applied Microbiology

Cell-free biomanufacturing

Bradley C. Bundy et al.

CURRENT OPINION IN CHEMICAL ENGINEERING (2018)

Review Biotechnology & Applied Microbiology

Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products

Jian Li et al.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2018)

Article Biochemical Research Methods

Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics

Richard Kelwick et al.

SYNTHETIC BIOLOGY (2018)

Article Biotechnology & Applied Microbiology

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

Matthew Deaner et al.

METABOLIC ENGINEERING (2017)

Article Multidisciplinary Sciences

A synthetic biochemistry platform for cell free production of monoterpenes from glucose

Tyler P. Korman et al.

NATURE COMMUNICATIONS (2017)

Article Biochemical Research Methods

Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool

Simon J. Moore et al.

BIOTECHNOLOGY JOURNAL (2017)

Article Biotechnology & Applied Microbiology

Establishing a high yielding streptomyces-based cell-free protein synthesis system

Jian Li et al.

BIOTECHNOLOGY AND BIOENGINEERING (2017)

Review Biotechnology & Applied Microbiology

Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production

Soo-Jung Kim et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2017)

Review Biochemistry & Molecular Biology

Engineering Cellular Metabolism

Jens Nielsen et al.

Article Biotechnology & Applied Microbiology

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

Ashty S. Karim et al.

METABOLIC ENGINEERING (2016)

Review Biotechnology & Applied Microbiology

Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications

Gang Wu et al.

TRENDS IN BIOTECHNOLOGY (2016)

Article Multidisciplinary Sciences

Engineering yeast for high-level production of stilbenoid antioxidants

Mingji Li et al.

SCIENTIFIC REPORTS (2016)

Article Biotechnology & Applied Microbiology

Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis

Jennifer A. Schoborg et al.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2016)

Article Biochemistry & Molecular Biology

Energizing eukaryotic cell-free protein synthesis with glucose metabolism

Mark J. Anderson et al.

FEBS LETTERS (2015)

Article Biotechnology & Applied Microbiology

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol

Jennifer E. Kay et al.

METABOLIC ENGINEERING (2015)

Article Multidisciplinary Sciences

SYNTHETIC BIOLOGY Complete biosynthesis of opioids in yeast

Stephanie Galanie et al.

SCIENCE (2015)

Article Multidisciplinary Sciences

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

Yong-Chan Kwon et al.

SCIENTIFIC REPORTS (2015)

Review Biochemical Research Methods

Cell-free metabolic engineering: Biomanufacturing beyond the cell

Quentin M. Dudley et al.

BIOTECHNOLOGY JOURNAL (2015)

Article Biotechnology & Applied Microbiology

Metabolic engineering of Saccharomyces cerevisiae for itaconic acid production

John Blazeck et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

Article Biotechnology & Applied Microbiology

Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis

Alaksh Choudhury et al.

BIOCHEMICAL ENGINEERING JOURNAL (2014)

Article Biotechnology & Applied Microbiology

Overproduction of Fatty Acids in Engineered Saccharomyces cerevisiae

Xiaowei Li et al.

BIOTECHNOLOGY AND BIOENGINEERING (2014)

Review Microbiology

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

Chris J. Paddon et al.

NATURE REVIEWS MICROBIOLOGY (2014)

Article Biochemistry & Molecular Biology

Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures

Waleed Ahmad Khattak et al.

PROCESS BIOCHEMISTRY (2014)

Article Biotechnology & Applied Microbiology

Optimized Extract Preparation Methods and Reaction Conditions for Improved Yeast Cell-Free Protein Synthesis

C. Eric Hodgman et al.

BIOTECHNOLOGY AND BIOENGINEERING (2013)

Review Green & Sustainable Science & Technology

Glycerol production and its applications as a raw material: A review

H. W. Tan et al.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2013)

Review Microbiology

Biochemistry of microbial itaconic acid production

Matthias G. Steiger et al.

FRONTIERS IN MICROBIOLOGY (2013)

Article Biochemical Research Methods

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Jian Ye et al.

BMC BIOINFORMATICS (2012)

Review Biochemistry & Molecular Biology

Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries

Kuk-Ki Hong et al.

CELLULAR AND MOLECULAR LIFE SCIENCES (2012)

Article Biotechnology & Applied Microbiology

Cell-free synthetic biology: Thinking outside the cell

C. Eric Hodgman et al.

METABOLIC ENGINEERING (2012)

Article Biotechnology & Applied Microbiology

Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering

Chiam Yu Ng et al.

MICROBIAL CELL FACTORIES (2012)

Article Biotechnology & Applied Microbiology

Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae

Jens Nielsen et al.

FEMS YEAST RESEARCH (2008)

Review Microbiology

Progress in metabolic engineering of Saccharomyces cerevisiae

Elke Nevoigt

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS (2008)

Article Multidisciplinary Sciences

Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae

G. N. Vemuri et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Review Biotechnology & Applied Microbiology

Expanding the metabolic engineering toolbox: more options to engineer cells

Keith E. Tyo et al.

TRENDS IN BIOTECHNOLOGY (2007)

Review Microbiology

Metabolic engineering of Saccharomyces cerevisiae

S Ostergaard et al.

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS (2000)