Journal
STAR PROTOCOLS
Volume 2, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100631
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Funding
- State Research Agency (AEI) from the Spanish Ministry of Science and Innovation [SAF2016-76598-R, SAF2017-92733-EXP, RTI2018-094834-B-I00, RED2018-102723-T]
- Spanish National Research and Development Plan
- Instituto de Salud Carlos III
- ERDF [PI17/02303, DTS19/00111, BIO2017-91272-EXP]
- Asociacion Espanola Contra el Cancer (AECC)
- Spanish Ministry of Science and Innovation
- AECC
- Health Institute Carlos III (ISCIII)
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Chromosomal instability (CIN) is a type of genomic instability that is associated with the progression of various diseases, including cancer. An optimized molecular cytogenetic protocol has been developed to detect and analyze CIN, providing a useful tool for cancer diagnosis and treatment.
Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).
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