4.1 Article

Detection of homolog-independent meiotic DNA repair events in C. elegans with the intersister/ intrachromatid repair assay

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STAR PROTOCOLS
Volume 2, Issue 3, Pages -

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ELSEVIER
DOI: 10.1016/j.xpro.2021.100801

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Funding

  1. National Institutes of Health [T32GM007413, R25HD070817, R00HD076165, R35GM128890]
  2. Advancing Science in America (ARCS) Foundation Award
  3. National Institutes of Health Office of Research Infrastructure Programs [NIH] [P40 OD010440]

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Accurate repair of DNA double-strand breaks in developing germ cells is crucial for proper chromosome segregation and genome integrity maintenance. Through utilizing the genetics and germline physiology of C. elegans, we successfully detected homolog-independent meiotic DSB repair and assessed recombination mechanisms.
Accurate repair of DNA double-strand breaks (DSBs) in developing germ cells is critical to promote proper chromosome segregation and to maintain genome integrity. To directly detect homolog-independent (intersister/intrachromatid) meiotic DSB repair, we exploited the genetics and germline physiology of C. elegans to (1) induce a single DSB in nuclei across discrete stages of meiotic prophase I; (2) detect repair of that DSB as a homolog-independent crossover or noncrossover; and (3) sequence the resultant product to assess mechanisms of recombination.For complete details on the use and execution of this protocol, please refer to Toraason et al. (2021).

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