Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems

Microscopy-based analysis of protein accumulation at a given subcellular loca-tion in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding yeast Saccharomyces cer-evisiae and fission yeast Schizosaccharomyces pombe. This protocol can be adapted for the analysis of any protein involved in any process as long as the pro-tein is localized to a discrete region of the cell. For complete details on the use and execution of this protocol, please refer to Okada et al. (2021) and Okada et al. (2019).

Automated summary

The protocol described offers a method for analyzing protein accumulation at a specific subcellular location in real time using microscopy, applicable to any protein involved in any cellular process. Researchers can refer to the studies by Okada et al. for full details on protocol usage and execution.