Journal
STAR PROTOCOLS
Volume 2, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100508
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Funding
- Fonds voor Wetenschappelijk Onderzoek (FWO) [MSCA: 754513]
- Aarhus University Research Founda- tion
- Lundbeckfonden [R307-2018-3667]
- Carlsberg Fonden [CF19-0687]
- Riisfort Fonden
- Steno Diabetes Center Aarhus
- University of Antwerp
- Strategisch Basisonderzoek Fonds voor Wetenschappelijk Onderzoek-Vlaanderen (SB-FWO)
- Marie Curie-IEF Fellowship
- Flemish Government, Fonds Wetenschappelijk onderzoek (FWO) , Founda- tion Against Cancer [2016-078]
- European Research Council (ERC) Advanced Research Grant [EU- ERC743074]
- Novo Nordisk Foundation
- ERC [ERC-713758]
- VIB TechWatch
- [NNF19OC0055802]
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Endothelial cells (ECs) have distinct phenotypical and functional characteristics depending on their tissue localization, playing crucial roles in diseases of various organs. Rapid and reproducible purification of blood vessel ECs can be achieved through mechanical and enzymatic digestion, providing important tools for research.
Endothelial cells (ECs) harbor distinct phenotypical and functional characteristics depending on their tissue localization and contribute to brain, eye, lung, and muscle diseases such as dementia, macular degeneration, pulmonary hyperten-sion, and sarcopenia. To study their function, isolation of pure ECs in high quan-tities is crucial. Here, we describe protocols for rapid and reproducible blood vessel EC purification established for scRNA sequencing from murine tissues us-ing mechanical and enzymatic digestion followed by magnetic and fluorescence -activated cell sorting. For complete details on the use and execution of these protocol, please refer to Kalucka et al. (2020), Rohlenova et al. (2020), and Goveia et al. (2020).
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