Journal
STAR PROTOCOLS
Volume 2, Issue 3, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100635
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Funding
- Australian National Health and Medical Research Council (NHMRC)
- AINSE Early Career Research Grant
- NHMRC Senior Research Fellowship [1159272]
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This protocol outlines the expression, purification, and crystallization of HLA-A*02:01 in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein, providing a method for visualizing viral peptides presented by HLAs for understanding T-cell responses.
Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A*02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides.For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).
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