Journal
ONCOIMMUNOLOGY
Volume 10, Issue 1, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/2162402X.2021.1969767
Keywords
gADC; TME; TIBs; TLSs; MALT
Categories
Funding
- National Natural Science Foundation of China [82060438]
- Future Science and Technology Star Project of Nanjing First Hospital
- Natural Science Foundation of Inner Mongolia Autonomous Region [2020MS08002]
- Fundamental Research Funds for the Central Universities [2632020ZD13]
- Academician workstation [YSZ2018-2]
- Doctoral Research Station of Bayannur [BYZ2019-1-2]
- Inner Mongolia Autonomous Region Science and Technology Plan Project [2020GG0273, 201802138]
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The study identified a substantial presence of MALT-B cells and NKT cells in gADC tissues, with MALT-B cells characterized by high expression of complement pathway activation genes.
The occurrence and development of gastric adenocarcinoma (gADC) is closely related to the interaction between tumor cells and immune cells in the tumor microenvironment (TME). Our objective was to characterize the repertoire of immune cells in the TME of gADC. To analyze the transcriptomic, immune, and spatial information of TME in gADC, we constructed single-cell RNA sequencing, 10 x Genomics V(D)J analysis, multiple immunofluorescence techniques, and OSCmap analysis of 49,765 single cells in seven samples from four gADC patients. Our integrative analysis of B cells demonstrated that a large number of mucosal associated lymphoid tissue (MALT)-B cells were detected in the gADC tissues, which have mature tertiary lymphatic structures (mTLSs), and almost no MALT-B cells in peripheral blood sample. Moreover, MALT-B cells are a class of IgA(+) plasma cells, which are characterized with high expression of complement pathway activation-related genes. Next, natural killer T (NKT) cells mainly exist in gADC tissues accompanied by mTLSs. This study also classified monocytes/macrophages and epithelial cells into benign and malignant types. Interestingly, CSOmap (q < .05) and multiple immunofluorescence (p < .05) results indicated more types of immune cells can be enriched in tissues with mTLSs than normal TLSs, and the density of mTLSs were higher than normal TLSs. Our findings provide novel insights for the signature of immune cells and tumor cells in the TME of gADC with TLSs and highlight the potential importance of IgA-mediated humoral immunity in gADC patients with TLSs.
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