Journal
JOURNAL OF NEMATOLOGY
Volume 53, Issue -, Pages -Publisher
SOC NEMATOLOGISTS
DOI: 10.21307/jofnem-2021-079
Keywords
D2-D3 of 28S rRNA gene; ITSrRNA gene; Description; Pinus virginiana; Pin nematode; Morphology; Morphometrics; Phylogeny; Scanning electron microscopy; Virginia pine
Categories
Funding
- U.S. Department of Energy
- USDA-ARS
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A new pin nematode species, Paratylechus beltsvillensis n. sp., was described and illustrated from rhizosphere soil of a Virginia pine tree in Beltsville, Prince George's County, Maryland, USA. Morphological and molecular analyses confirmed the presence of this new species in the soil samples from Beltsville Park.
The pin nematode, Paratylechus beltsvillensis n. sp. collected from rhizosphere soil of a Virginia pine tree (Pinus virginiana Mill) growing in Little Paint Branch Park, Beltsville, Prince George's County, Maryland, USA, is described and illustrated along with light and scanning electron photomicrographs. Females, males, and juveniles of this new species were recovered from soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Morphologically, females are short, body length ranging from 245 to 267 mu m, stylet from 70 to 75 mu m long with anchor shaped knobs, vulva located at 70-73% and small vulval flap, spermatheca large, and ovoid filled with sperms. Lateral field with three incisures, of which the outer two are prominent. Tail slender, having a rounded tail terminus. Males without stylet and have a degenerated pharynx, spicules =17-20 mu m and gubernaculum=5.0-5.5 mu m. Both morphological observations and molecular analysis of ITS and partial 28S ribosomal RNA gene sequences indicated that the specimens collected from the soil at Beltsville Park from rhizosphere soil samples from Virginia pine represents a new pin nematode species.
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