The spatial distribution of the photosynthetic picoeukaryotes community structure in Lake Hongze

The spatial dynamics of picophytoplankton were investigated by flow cytometry and high-throughput sequencing in Lake Hongze, a large river-connecting lake. Picophytoplankton were mainly composed of phycocyanin-rich picocyanobacteria (PCY) and photosynthetic picoeukaryotes (PPEs). Picocyanobacteria was the dominant picophytoplankton group since the contribution of PPEs to total picophytoplankton was only 11.78%. However, PPEs were highly diverse and were composed of Chlorophyta, Bacillariophyta and Chrysophyceae. Environmental factors showed spatial differences, particularly in total phosphorus (TP), suspended solids (SS) and chemical oxygen demand (CODMn), which showed relatively high concentrations around the river channel. The abundances of PPEs and PCY showed similar spatial patterns, which were relatively low in the river course since they were negatively related to SS and CODMn. The top 10 OTUs contributed 79.18% of the total PPEs reads and affiliated with main species in PPEs. CCA results showed that, PPEs community composition was significantly impacted by temperature and DO at sites away from river channel, while was only influenced by nitrite at sites around the river channel.

Automated summary

The spatial dynamics and composition of picophytoplankton in Lake Hongze were investigated using flow cytometry and high-throughput sequencing. Picocyanobacteria dominated the community, while photosynthetic picoeukaryotes were diverse but less abundant. Environmental factors, particularly total phosphorus, suspended solids, and chemical oxygen demand, showed spatial differences with higher concentrations near the river channel. The abundances of different picophytoplankton groups exhibited similar spatial patterns, being negatively related to certain environmental factors like suspended solids and chemical oxygen demand.