Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides

Development of the methods to examine the molecular targets of biologically active compounds is one of the most important subjects in experimental biology/biochemistry. To evaluate the usability of the (7-nitro-2,1,3-benzoxadiazole)-thioether (NBD-S) probe for this purpose, bioactive chemical probe (1) as the cellulose biosynthesis (CB) inhibitor was synthesized and tested. As a result, a variety of fluorescently-labeled particles and organelles were found in the columella root cap cells of radish plants. Of note, well-defined cellular organelles were clearly recognized in the detaching root cap cells (border-like cells). These results imply that the bioactive NBD-S chemical probe could be a valuable direct-labeling reagent. Analysis of these fluorescent substances would be helpful in providing new information on defined molecular targets and events.

Automated summary

The development of methods to examine the molecular targets of biologically active compounds is a crucial subject in experimental biology and biochemistry. The usability of the NBD-S probe as a direct-labeling reagent was evaluated by synthesizing a bioactive chemical probe to inhibit cellulose biosynthesis. The results showed various fluorescently-labeled particles and organelles in root cap cells, particularly recognizably in detaching root cap cells, suggesting valuable information for studying molecular targets and events.