Element probe based CRISPR/Cas14 bioassay for non-nucleic-acid targets

Herein, we propose an element probe based CRISPR/Cas14 detection platform and apply it to the detection of non-nucleic-acid targets. Combining metal isotope detection and CRISPR/Cas14 biosensing, the sensitive detection of non-nucleic-acid targets could be realized. We designed and optimized the element probe, which proved that Cas14 has a preference for longer lengths in element probe cleavage. Using this method, the quantitative detection of trace aqueous ampicillin can be achieved within 45 minutes at room temperature (25 degrees C). A detection limit as low as 2.06 nM is obtained with excellent performance in anti-interference tests and complex matrix detection.

Automated summary

A novel element probe based CRISPR/Cas14 detection platform was proposed in this study, allowing sensitive and quantitative detection of non-nucleic-acid targets such as trace aqueous ampicillin within 45 minutes at room temperature. The method showed excellent performance in anti-interference tests and complex matrix detection, with a low detection limit of 2.06 nM.