Biological behavior of nanoparticles with Zr-89 for cancer targeting based on their distinct surface composition

Nano-sized materials with properties that enable their internalization into target cells using passive targeting systems have been utilized with radioisotopes to track their pharmacokinetics in the body. Here, we report the incorporation of novel chelator-free Zr-89 using a hierarchical iron oxide nanocomposite (Zr-89-IONC). Characterization revealed that it had a rice-shape with a mean width of 160 nm. The surface of the Zr-89-IONCs was coated by polyethyleneimine (PEI) and polyvinylpyrrolidone (PVP) to improve the cancer target efficacy. The biological behavior of the nanoparticles coated with the polymers differed significantly by the surface composition. Positron emission tomography measurements by the labeled Zr-89 effectively confirmed the cancer target capability and the fate of distribution in the body. We found that only PVP coated Zr-89-IONC reached the tumor region while non-coated and PEI coated Zr-89-IONC tended to be undesirably entirely cleared in the liver and spleen. The Zr-89-incorporated iron oxide nanocomposite is significantly stable for radiolabeling despite various surface modifications, allowing the potential carrier to specifically target cancer cells. The strategy of utilizing the biocompatible PEI and PVP surface coating system for negative charged nanoparticles such as iron oxide will afford enhanced biological application.

Automated summary

A novel chelator-free Zr-89-IONC with rice-shaped morphology was developed and coated with polyethyleneimine (PEI) and polyvinylpyrrolidone (PVP) to improve cancer targeting efficacy. Positron emission tomography measurements confirmed that only PVP-coated Zr-89-IONC reached the tumor region, while non-coated and PEI coated ones tended to be cleared in the liver and spleen. The Zr-89-incorporated iron oxide nanocomposite showed stability for radiolabeling, making it a potential carrier for specific cancer cell targeting.