Journal
ACS APPLIED BIO MATERIALS
Volume 4, Issue 9, Pages 6832-6842Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.1c00544
Keywords
conjugated dextran; axonal transport; retrograde tracing; anterograde tracing; DLS; SANS
Funding
- University of Oslo Life Science Program
- Norwegian ALS patient advocacy group ALS-Norge
Ask authors/readers for more resources
The study found that dextran conjugates with lysine and TRITC of different molecular weights exhibited variations in molecular volumes and mobility within axons. Surprisingly, lower molecular weight conjugated dextrans showed higher efficiency in translocation despite having larger molecular volumes compared to higher molecular weight dextrans.
As a potent nonviral system for biomolecular delivery to neurons via their axons, we have studied molecular characteristics of lysinated fluorescent dextran nanoconjugates with degrees of conjugation of 0.54-15.2 mol lysine and 0.25-7.27 mol tetramethyl rhodamine isothiocyanate (TRITC) per mol dextran. We studied the influence of conjugation with lysine and TRITC on the size and structure of different molecular weight dextrans and their mobility within axons. Dynamic light scattering (DLS) and small-angle neutron scattering (SANS) experiments revealed significant differences in the size and structure of unmodified and modified dextrans. Unexpectedly, lower-molecular-weight conjugated dextrans exhibited higher molecular volumes, which we propose is due to fewer intramolecular interactions than in higher-molecular-weight conjugated dextrans. Assessment of retrograde and anterograde movement of lysine- and TRITC-conjugated dextrans in axons in the lumbar spinal cord of chicken embryos showed that lower-molecular-weight dextrans translocate more efficiently than higher-molecular-weight dextrans, despite having larger molecular volumes. This comparative characterization of different molecular weight dextrans will help define optimal features for intracellular delivery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available