Journal
BIOENGINEERED
Volume 12, Issue 1, Pages 7552-7562Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.1979914
Keywords
Pulpitis; S14G-humanin; dental pulp cells; NF-kappa B
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Funding
- Changzhi Pandeng Project [H30280036]
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The study found that S14G-humanin protected LPS-treated human dental pulp cells by inhibiting the TLR4/MyD88/NF-kappa B signaling pathway.
Pulpitis is reported in large populations of patients and significantly impacts their normal life quality. It is reported that the lipopolysaccharide (LPS) in Gram-negative bacteria induces severe inflammation in dental pulp tissues. S14G-humanin is a derivative of humanin and has been recently confirmed to possess promising anti-inflammatory properties. The current study aims to explore the possibility of treating pulpitis with S14G-humanin. LPS-stimulated dental pulp cells (DPCs) were utilized to simulate an inflammatory state in the progression of pulpitis. We found the elevated expressions and production of interleukin- 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), macrophage chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), upregulated Pentraxin 3 (PTX3) and activated oxidative stress in LPS-treated DPCs were all reversed by treatment with 50 and 100 mu M S14G-humanin. In addition, the LPS-induced elevated expression levels of toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (Myd88), and activation of the I kappa B alpha/NF-kappa B signaling pathway in hDPCs were significantly repressed by treatment with S14G-humanin. Conclusively, we found that S14G-humanin protected LPS-treated hDPCs by inhibiting the TLR4/MyD88/NF-kappa B signaling pathway.
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