4.7 Article

Effects of dexmedetomidine on postoperative cognitive function of sleep deprivation rats based on changes in inflammatory response

Journal

BIOENGINEERED
Volume 12, Issue 1, Pages 7920-7928

Publisher

TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.1981757

Keywords

Sleep deprivation; dexmedetomidine; inflammatory response; cognitive function; rat

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The study aimed to evaluate the effects of DEX on the postoperative cognitive function of SD rats, and findings suggest that DEX can effectively alleviate postoperative hippocampal inflammation and improve cognitive function in SD rats. The mechanism may be related to the regulation of the Rac1/AKT/NF-κB pathway by DEX, leading to reduced oxidative stress in hippocampal neurons and restoration of damaged cells.
We aimed to assess the effects of dexmedetomidine (DEX) on postoperative cognitive function of sleep deprivation (SD) rats based on changes in inflammatory response. Male rats were randomly divided into blank control (C), SD, DEX, and SD+DEX groups. The SD model was established through intraperitoneal injection of DEX. The escape latency was detected through Morris water maze test daily, and the mechanical withdrawal threshold and thermal withdrawal latency were detected for 8 d. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in hippocampus homogenate were determined, and the morphological changes in neurons were detected through Nissl staining. The concentration of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and IL-6 in the hippocampus was detected by enzyme-linked immunosorbent assay, and the Rac1/protein kinase B (AKT)/nuclear factor-kappa B (NF-kappa B) expressions were detected by Western blotting. The changes in immunofluorescence localization of NF-kappa B were observed by confocal microscopy. Compared with SD group, the escape latency was shortened, original platform-crossing times increased, MDA content declined, SOD activity rose, neurons were arranged orderly and number of Nissl bodies increased in the hippocampal CA1 region, levels of IL-1 beta, TNF-alpha, and IL-6 in the hippocampus decreased, Rac1/AKT/NF-kappa B expressions were down-regulated, and proportion of NF-kappa B entering the nucleus declined in SD+DEX group (P < 0.05). DEX can effectively alleviate postoperative hippocampal inflammation and improve cognitive function of SD rats. The ability of DEX to relieve oxidative stress of hippocampal neurons, restore damaged cells, and reduce hippocampal inflammation in SD rats may be related to the Rac1/AKT/NF-kappa B pathway.

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