4.0 Article

Measuring Total Classical Pathway and Activities of Individual Components of the Mouse Complement Pathway

Journal

BIO-PROTOCOL
Volume 11, Issue 19, Pages -

Publisher

BIO-PROTOCOL
DOI: 10.21769/BioProtoc.4175

Keywords

Complement; Haemolytic; Assay; Classical; CH50; Depleted sera; Mouse

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Funding

  1. Health and Care Research Wales Fellowship from Welsh Government

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The complement system plays a crucial role in innate immunity by recognizing and killing bacteria through opsonization, phagocytosis, and direct lysis. Measuring complement activity in mouse serum is challenging and typically requires a large amount of serum, while utilizing human sera depleted of single complement proteins can be a helpful alternative.
The complement system is a central component of innate immunity, responsible for recognition and killing of bacteria by tagging invaders through opsonisation, thereby promoting phagocytosis, and by direct lysis. Complement activity is routinely measured using functional assays that utilise erythrocytes as targets. The classical pathway haemolytic assay (CH50) with antibody sensitised sheep erythrocytes as target is used worldwide in clinical and research laboratories to measure complement activity in human and rodent sera. While there are no particular limitations in the human assay, measuring complement in mouse serum is more difficult and usually requires large amounts of serum, which is challenging to collect in experiments. In particular, it is challenging to measure the activities of individual mouse complement proteins. To overcome this hurdle, we have developed protocols that employ human sera depleted of single complement proteins as the source of the other complement proteins and test mouse serum to restore the relevant component. This simple haemolytic assay is a useful tool for confirming natural or engineered complement deficiencies and complement dysregulation in mouse models.

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