Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 36, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2105510118|1of11
Keywords
membrane trafficking; endosome; endosomal sorting; sorting nexin; SNX27
Categories
Funding
- Natural Science Foundation of China [91854121, 31871429]
- National Key Research and Development Program of China [2018YFC1005004]
- Sichuan Science and Technology Program [2018RZ0128]
- 1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University [ZYGD20007, ZYJC18011]
- US NIH [DK107733]
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This study reveals a functional distinction between the FERM domain of SNX27 and SNX17, showing that SNX27 interacts with a novel DLF motif in SNX1/2 instead of the NPxY/NxxY motif in cargo proteins. The interaction between SNX27 and SNX1/2 is critical for efficient recruitment of SNX27 to endosomes and endocytic recycling of multiple cargoes, and is also crucial for brain development in zebrafish. The findings suggest that SNX27 and SNX17 mediate endocytic recycling through fundamentally distinct mechanisms.
The molecular events that determine the recycling versus degradation fates of internalized membrane proteins remain poorly understood. Two of the three members of the SNX-FERM family, SNX17 and SNX31, utilize their FERM domain to mediate endocytic trafficking of cargo proteins harboring the NPxY/NxxY motif. In contrast, SNX27 does not recycle NPxY/NxxY-containing cargo but instead recycles cargo containing PDZ-binding motifs via its PDZ domain. The underlying mechanism governing this divergence in FERM domain binding is poorly understood. Here, we report that the FERM domain of SNX27 is functionally distinct from SNX17 and interacts with a novel DLF motif localized within the N terminus of SNX1/2 instead of the NPxY/NxxY motif in cargo proteins. The SNX27-FERM-SNX1 complex structure reveals that the DLF motif of SNX1 binds to a hydrophobic cave surrounded by positively charged residues on the surface of SNX27. The interaction between SNX27 and SNX1/2 is critical for efficient SNX27 recruitment to endosomes and endocytic recycling of multiple cargoes. Finally, we show that the interaction between SNX27 and SNX1/2 is critical for brain development in zebrafish. Altogether, our study solves a long-standing puzzle in the field and suggests that SNX27 and SNX17 mediate endocytic recycling through fundamentally distinct mechanisms.
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