Journal
METHODSX
Volume 8, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mex.2021.101243
Keywords
Solubility; Hydrophobic interactions; Disulfide bonds; Electrostatic interactions; Sodium dodecyl sulfate; Dithiothreitol
Categories
Funding
- IGF Projects of the FEI (AiF) [20197 N, 19712 N]
- Industrial Collective Research (IGF) of the German Ministry of Economic Affairs and Energy (BMWi)
Ask authors/readers for more resources
Understanding the stabilizing protein interactions in protein gels is crucial for both food and biotechnology applications. There are two main methods currently used, with the second method being preferred due to its ability to cleave interactions without altering the gelling mechanism. Existing methods are often limited to specific gel systems, but a new method introduced in this paper is suitable for highly denatured whey and plant proteins.
Understanding the stabilizing protein interactions in protein gels is of high importance for food- and biotechnology. Protein interactions in protein gels can help to predict hardness, deformability and other gel parameters. Currently there are two types methods used. One is to use protein interaction blocking agents and the other is to dissolve the gel in different buffer systems, which cleave the interactions. The first method alters the gelling mechanism, which is why the second method is the preferred one. However, currently published methods are often only suitable for specific gel systems as for example weakly bound protein gels. In this paper, a method is introduced, which is suitable for highly denatured whey and plant protein. Suitable for strongly cross-linked whey protein and plant protein gels Stronger buffer system to ensure cleavage of all protein interactions More reproducible and simplified crushing of the gel without the introduction of uncontrolled shear stress excessively affecting the analysis of chemical bonds (C) 2021 The Author(s). Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available