Journal
METHODSX
Volume 8, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mex.2021.101422
Keywords
Biotechnology; Fish sperm quality; Fish sperm morphology; Fish sperm centrifugation; Teleost
Categories
Funding
- CAPES -Coordination for the Improvement of Higher Education Personnel [CAPES/PROEX N DEG, 88882.346090/2015-01]
- UFRGS Embryology and Reproduction Bioethics Laboratory
Ask authors/readers for more resources
Density gradient centrifugation is a technique used to wash or separate cryopreserved sperm samples, allowing for the reduction of cryoprotectant concentration and obtaining a better quality fraction of sperm. The study compared different centrifugation times and forces, with the method at 1000 x g for 10 min proving to yield a better fraction of normal sperm.
Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm. The proposed method seeks to reduce the presence of cryoprotectant in the cryopreserved milt of the Rhamdia qhelen and to obtain a fraction of better quality sperm. Gradient centrifugation was formed from 90% AllGrad (R) and different centrifugation times and forces were compared. The separated sperm presented a low increase in motility and decreased head damage and presence of gout, however, it was better compared to the non-separated samples. The speed of 1000 x g for 10 min, 4 degrees C, allowed 22.25 +/- 4.64% of normal spermatozoa, that is, 9.25% more than the non-centrifuged milt (p = 0.0013). The centrifugation method allows a fraction of spermatozoa morphologically less affected by cryopreservation. Density gradient centrifugation with AllGrad (R) 90% is proposed as a tool of easy adaptation and application for the separation of cryopreserved sperm of R. quelen. Density gradient centrifugation method at 1000 x g for 10 min allows obtaining a better fraction of normal sperm. (C) 2021 The Author(s). Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available