Ultrasound-Assisted Emulsified Microextraction Based on Deep Eutectic Solvent for Trace Residue Analysis of Metribuzin in Urine Samples

Deep eutectic solvent was used as extraction solvent to develop and optimize a new sample preparation method for the determination of metribuzin in urine samples. In order to determine the optimal values of the effective factors in the deep eutectic solvent-based ultrasound assisted emulsification microextraction method, six effective parameters were selected. The design of experiments was performed using the one-variable-at-a-time method. Totally, 96 experimental runs were performed, and the samples were analyzed using high-performance liquid chromatography with a UV detector. Under the optimum conditions, the calibration curve for metribuzin was linear in the concentration range of 5 to 500 mu g L-1 for urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR %) and relative standard deviation (RSD %) tests on the fortified urine samples. RR % and RSD % were found to be 96.3 - 101.7 % and 3.2 - 7.6 %, respectively. The limit of quantification and the limit of detection were obtained 5 and 0.8 mu g L-1, respectively.

Automated summary

A new sample preparation method using deep eutectic solvent for determining metribuzin in urine samples was developed and optimized. The method showed linear calibration curve, high accuracy, and reproducibility under the optimum conditions. With a limit of quantification of 5 μg L-1, the method demonstrated good sensitivity for detecting metribuzin.