Ultraviolet photodissociation circular dichroism spectroscopy of protonated L-phenylalanyl-L-alanine in a cryogenic ion trap

We obtained ultraviolet photodissociation (UVPD) circular dichroism (CD) spectra of protonated L-phenylalanyl-L-alanine (L-H(+)PheAla) near the origin band of the S-0-S-1 transition using cryogenic ion spectroscopy. Infrared (IR) ion-dip, IR-UV hole burning (HB) and UV-UV HB spectra showed that L-H(+)PheAla existed as two different conformers in a cryogenic ion trap, and they had nearly identical peptide backbones but different conformations in the Phe side chain. The UVPD CD spectra revealed that the two conformers had opposite CD signs and significantly different CD magnitudes from each other. These results demonstrate that the CD value of l-H(+)PheAla near the origin band is strongly influenced by the conformation of the Phe side chain.

Automated summary

Through ultraviolet photodissociation circular dichroism spectra, it was found that protonated L-phenylalanyl-L-alanine existed as two different conformers in a cryogenic ion trap, with opposite CD signs and significantly different CD magnitudes near the origin band. This demonstrates the strong influence of the conformation of the Phe side chain on the CD value of the compound.