4.7 Article

Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Journal

CHEMICAL COMMUNICATIONS
Volume 57, Issue 91, Pages 12155-12158

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc05181d

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Funding

  1. King Mongkut's University
  2. Research Network NANOTEC (RNN) program of the National Nanotechnology Center (NANOTEC), NSTDA, Ministry of Science and Technology, Thailand [P1851883]

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The study presents a highly sensitive and selective multiplex assay utilizing an electrochemical DNA sensor and isothermal rolling circle amplification to detect and discriminate three common entero-pathogens in a single reaction. The assay has femtomolar sensitivity and is particularly useful for field- or resource-limited settings.
We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.

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