Journal
ANALYST
Volume 146, Issue 23, Pages 7230-7239Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1an01525g
Keywords
-
Categories
Ask authors/readers for more resources
A novel microfluidic device was designed to efficiently separate sperm cells without the need for tedious sample processing and centrifugation steps. The device utilizes inertial focusing and hydrodynamic filtration techniques to achieve cell separation in multiple micro-slits. Experimental results showed a separation efficiency of approximately 76% in simulated samples.
A triplet spiral channel coupled with cross-flow filtration has been designed and fabricated in an effort to separate sperm cells from either semen or simulated testicular sperm extraction (TESE) samples. This device separates a fraction of cells from the sample by taking advantage of inertial focusing combined with hydrodynamic filtration in multiple micro-slits. Compared to the conventional swim-up technique, the proposed microfluidic device is capable of efficiently separating sperm cells without any tedious semen sample processing and centrifugation steps with a lower level of reactive oxygen species and DNA fragmentation. The device processing capability on the simulated TESE samples confirmed its proficiency in retrieving sperm cells from the samples with an approximate yield of 76%. Conclusively, the introduced microfluidic device can pave the path to proficiently separate sperm cells in assisted reproductive treatment cycles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available