Journal
IN VIVO
Volume 35, Issue 6, Pages 3181-3191Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/invivo.12613
Keywords
Curcumin derivative; MTH-3; palmitic acid; insulin resistance; mouse myoblast cells
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Funding
- China Medical University Hospital [DMR-110-155]
- China Medical University [CMU103-S-16, MOST 106-2314-B-039-046]
- Taipei Veterans General hospital [V110B-038]
- Yen Tjing Ling Medical Foundation [CI-110-6]
- Chinese Medicine Research Center, China Medical University from The Featured Areas Research Center Program within the Ministry of Education (MOE) in Taiwan, R.O.C.
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The study showed that MTH-3 could reduce oil droplet production induced by PA and promote glucose uptake, potentially becoming an effective drug for insulin resistance and related diseases.
Background/Aim: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. Materials and Methods: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2NBDG assay. Results: Treatment of cells with up to 500 mu M PA for 24 h or with 5 or 10 mu M MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. Conclusion: MTH3 may become an efficient drug for the treatment of insulin resistance and associated diseases.
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