Co-immunofluorescence of MRPL12 and Nrf2 in HK2 Cells

Immunofluorescence is a technique to visualize the localization of specific molecule targets within cells using the specificity of antibodies. Here, we describe a protocol to detect two different protein components in a cell simultaneously. Antibody concentrations to be used vary from cell to cell and should be optimized for different cell types. In this protocol, we perform co-immunofluorescence of mitochondrial ribosomal protein L7/L12 (MRPL12) and nuclear factor erythroid 2-related factor 2 (Nrf2), a potential transcription factor of MRPL12, in HK-2 cells, as an example. Taking advantage of the diverse set of antibodies raised in different species, we are able to analyze the colocalization and expression of these proteins.

Automated summary

Immunofluorescence is a technique for visualizing specific molecules in cells using antibodies; optimizing antibody concentrations for different cell types is necessary; a variety of antibodies from different species can be used to analyze protein co-localization and expression.