Investigating the in vitro hypoglycaemic and antioxidant properties of Citrus x clementina Hort. juice

Citrus x clementina juice obtained from fruits collected in three different areas (flood plain, hill and coastal plain) was investigated for the chemical composition, radical scavenging properties (DPPH and ABTS tests), and alpha-amylase and alpha-glucosidase inhibitory activity. Neohesperidin (72.96-116.50 mg/100 mL), hesperidin (55.24-69.52 mg/100 mL) and narirutin (7.21-12.13 mg/100 mL) are the main flavonoids identified by HPLC analyses. In carbohydrate hydrolysing enzymes inhibitory activity tests, samples showed higher potency against alpha-glucosidase. Juice from hill was the most active with an IC50 value of 77.79 mu g/mL. Data on the radical scavenging activity revealed the following trend of potency flood plain > coastal plain > hill. These results could help farmers to select fruits for different industrial purpose such as functional food and matrix to extract nutraceutical products.